Modaplex FGFR Gene Fusion Analysis Kit

The Modaplex FGFR Gene Fusion Analysis Kit is a PCR-based multiplex assay for the qualitative detection of gene fusions in FGFR1, FGFR2, and FGFR3. The kit provides all the reagents necessary for the rapid and sensitive detection of eight FGFR gene fusions. Together with the Modaplex technology, the kit provides trustworthy results, while using a workflow that is as simple as a standard PCR.

The Modaplex FGFR Gene Fusion Analysis Kit is intended for research use only purposes and not for diagnostic use.

Modaplex FGFR CNV Analysis Kit

Product Specification

Targets   BAG4:FGFR1 (e2:e6), BAG4:FGFR1 (e1:e8), FGFR2:CIT (e17:e23), FGFR3:TACC3 (e17:e4), FGFR3:TACC3 (e17:e8), FGFR3:TACC3 (e17:e10), FGFR3:TACC3 (e17:e11), FGFR3:TACC3 (e17:e4intra)
Kit Content   FGFR Fusion Primer, FGFR Fusion RT Primer, Taq DNA Polymerase, Buffer, Positive Control, Nuclease-free Water for NTC, Sample Dilution
Kit Size   50 reactions
Sample Material   Total human RNA extracted from FFPE tissue
RNA Input   12.5ng – 400ng
Reproducibility  

To assess the reproducibility of the FGFR Gene Fusion Analysis Kit, the following tests have been performed:

  • Intra-assay reproducibility
  • Kit and reagent lot-to-lot reproducibility
  • Inter-laboratory reproducibility
  • Inter-Modaplex reproducibility

The Modaplex FGFR Gene Fusion Analysis Kit has passed all tests with a call accuracy of 100%.

Technology   Modaplex
Result Interpretation   With Biotype Moda-RA software

Workflow

The Modaplex FGFR Gene Fusion Analysis Kit is to be used with the Modaplex technology, a bench-top system that integrates PCR-based amplification with automated capillary electrophoresis (CE)-based detection of amplification products. In combination with the Modaplex FGFR Gene Fusion Test and the analysis software Moda-RA, copy number variations can be assessed in just 3.5 hours.Modaplex Workflow

A universal PCR amplification profile allows the simultaneous analysis of various biological modalities. Thus, FGFR gene fusion analysis can be combined with any other test for FGFR/FGF genetic alterations in a single run. The workflow and the turnaround time remain unchanged. Therefore, the Modaplex technology is an ideal instrument for multi-analyte assays.

Order Information

Product: Modaplex FGFR Gene Fusion Analysis Kit
Cat. no.: BTI-C001-B1-2-0050

To order the FGFR Gene Fusion Analysis Kit, please e-mail us at sales@biotype.de. Let us know about the specific products you are looking for (incl. their catalog numbers) and we will send you an individual offer customized to your requirements.