MODAPLEX POLE/POLD1 Mutation
Analysis Kit
For improved decision-making:
A comprehensive POLE/POLD1 mutation panel
FEATURES
- Analyze 19 POLE and POLD1 mutations required for the risk classification of endometrial cancer
- Low DNA input – 4 ng per sample
- Benefit from an efficient workflow using a kit-specific data analysis software requiring no additional bioinformatics
- Verified with colorectal and endometrial cancer samples
Detection and differentiation of 19 somatic and rare germline mutations in the polymerase epsilon and polymerase delta-1 exonuclease domains is effectively achieved using the MODAPLEX POLE/POLD1 Mutation Analysis Kit. The assay enables researchers to advance promptly in clinical research.
SPECIFICATIONS
Cat. No. | Â | 85-10101-0050 |
Panel | Â |
POLE EDMs: T278M, P286L, P286H, P286R, S297A, S297F, F367S, V411L(G>C), V411L (G>T), H422N, L424V, P436R, M444K, A456P, S459F, A465V POLD1 EDMs: D316N, C319Y, S478N |
Reactions | Â | 1 Multiplex-PCR per sample |
Kit content | Â |
All reagents required to perform PCR are part of the MODAPLEX POLE/POLD1 Mutation Analysis Kit • POLE/POLD1 Primer Mix |
Internal controls | Â | 2 (POLE and POLD1 gene) |
PCR controls | Â | 2 included (PC, NTC) |
Sample input | Â | ~ 4 ng gDNA (FFPE) |
Sensitivity | Â | Down to 2 % |
Turn-around time | Â | ~ 4 h after nucleic acid preparation (including data analysis) |
Detection | Â | Qualitative |
To be used with | Â | MODAPLEX instrument |
Data analysis | Â | MODAPLEX Reporter software |
Workflow
The Modaplex POLE/POLD1 Mutation Analysis Assays is to be used with the Modaplex instrument platform version 1.0 or higher. Thus, the POLE/POLD1 workflow is similar to the workflow of other Biotype Modaplex assays such as MSI Analysis Assay and comprises the same three steps after nucleic acid preparation: PCR set up, Modaplex run, and result interpretation.
Downloads
Order Information
Product: | MODAPLEX POLE/POLD1 Mutation Analysis Kit |
Size | 50 reactions |
Cat. No. | 85-10101-0050 |
Status | RUO* |
*RUO – Research Use Only products must be validated by the customer with clinically relevant material for diagnostic purposes.
To order the MODAPLEX POLE/POLD1 Mutation Analysis Kit, please e-mail us at sales@biotype.de