MODAPLEX Technology

Workflow automation for modular multi-gene testing

The MODAPLEX System merges polymerase chain reaction (PCR) and capillary electrophoresis (CE) into one automated workflow. This technology merge enables the analysis of multiple molecular markers in a single workflow under routine settings. Each MODAPLEX PCR reaction consists of multiple primers and probes unique for a specific set of targets. Through high-level multiplexing and parallelized analysis of various genetic alterations (e. g. mutations, gene fusions, copy number variations), MODAPLEX allows every lab to detect biomarker signatures in a simple and cost-effective workflow.

During the PCR cycle, the fluorescently labeled PCR amplicons are continuously injected into the capillary gel. There, they are separated based on their physical properties and subsequently detected after passing through two lasers. This process is repeated over several PCR cycles during a MODAPLEX run, generating real-time data for each well and each target.